Designing primers using amplifx software#

Primer3 - a common used software for designing primers Primaclade - a web-based application that accepts a multiple species nucleotide alignment file as input and identifies a set of PCR primers that will bind across the alignment. PRIDE - The less automated webversion of PRIDE (a.o. Pira-PCR - PCR Primer design for restriction analysis of single nucleotide polymorphisms Osprey - Oligonucleotide Design Software for Sequencing and Gene Expression The oligodb system uses the human DNA-transcripts of ENSEMBL. Oligodb - a web-based system for interactive design of oligo DNA for transcription profiling (hybridization) of human genes. NetPrimer - Primer design service of Premier Biosoft. MPrime - an interface which allows the efficient detection of multiple primers or oligonucleotides for genic regions in either the human, mouse, rat, zebrafish, or fruit fly genomes. Methprimer - Design primers for methylation PCR MEDUSA - A tool for automatic selection and visual assessment of PCR primer pairs (Karolinska) Genefisher - Interactive PCR primer design GAP (Genome- wide Automated Primer finder servers) - Design thousands pairs of primers for amplifying a set of open reading frames over an entire genome. PCR Bioinformatics and Web based primer designĪntiSense Design - Design antisense primers at IDTĪutoPrime - designs primers that are specific for expressed sequences (mRNA).ĬODEHOP - COnsensus-DEgenerate Hybrid Oligonucleotide PrimersĮLXR - Exon Locator and eXtractor for Resequencing - Design exon amplification primers for a complete geneĮLXRdb - Exon Locator and eXtractor for Resequencing database - Retrieve precalculated primers for a complete geneĮxonprimer - Design primers for the amplification of exons with intronic primers Design of PCR and Sequencing Primers and PCR reactionsĭesign of Primers for Automated Sequencing

Designing primers using amplifx